日本植物病理学会報
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
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神奈川県で発生したスイカ灰白色斑紋ウイルス(WSMoV)の特性とキュウリでの病徴の再現
島田 涼子奥田 充植草 秀敏
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2019 年 85 巻 2 号 p. 108-111

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In 2016, malformation and spotted wilt of leaves and fruit malformation were observed on cucumber (Cucumis sativus) in Kanagawa Prefecture. RT-PCR to amplify an RNA dependent RNA polymerase gene region of tospovirus and sequencing of the amplified fragment indicated highest identity with watermelon silver mottle orthotospovirus (WSMoV). The virus was isolated through two rounds of single lesion isolation and identified as WSMoV based on the nucleocapsid gene sequence. Cucumber plants inoculated with the isolated virus developed the same symptoms, and only WSMoV was shown to be present in RNA from symptomatic plants; nine other cucumber viruses were absent. This is the first report describing WSMoV symptoms on cucumber.

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