Abstract
Antibody production in tumorous (Ehrlich's) ascites of mice injected with various antigens, tobacco mosaic virus (TMV), several strains of cucumber mosaic virus (CMV), and healthy tobacco protein, was investigated. Highest antibody titre of the mouse ascitic fluid was 1024-fold when injected with 3mg of TMV antigen mixed with equal volume of Freund's complete adjuvant. Ascitic fluids having antibody titre, 128 and 512-fold were also obtained from mice injected with 0.3mg and 1.75mg of CMV-O antigen, respectively. Maximum volume of ascitic fluid containing antibody obtained from single mouse was 38ml. From the results of the present experiments, It was concluded that three weekly injections of 0.1-1mg of antigen mixed with Freund's adjvant, followed by injection of tumor cells was convenient for obtaining a high content of antibody.
This technique will be useful for antibody production to some plant viruses which have poor yield and are difficult to purify.
