Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Application of Airbrush-Inoculation Technique to the Screening of Anti-Plant-Viral substances
II. Inoculation with CGMMV
Keng-Tang HUANGYasuhide MATSUZAWATomomasa MISATO
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1974 Volume 40 Issue 1 Pages 1-5

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Abstract
Spraying inoculation of CGMMV on cucumber seedlings was performed by automatic sprayer. When the spraying conditions (gauge pressure: 4kg/cm2, spraying distance: 20cm, exposure periods: 0.5sec. and carborundum conc.: 1%) were applied, the CGMMV inoculum, fresh weight (g) of infected leaves per 50-100ml of distilled water, was necessary for complete infection on 3 week-grown seedlings of cucumber plant (var. Sagami-Hanjiro).
These conditions were affected by each other. However, carborundum contents from 0.5 to 2% might be less affectible, whereas, 20% of carborundum content apparently enhanced the infectivity of CGMMV.
Several substances were tested for their anti-CGMMV activities, and none of them showed the remarkable inhibition, except that N-lauroyl-DL-methionine showed some-what weak inhibitory activities.
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© The Phytopathological Society of Japan
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