1999 年 43 巻 1 号 p. 100-104
There is increased awareness that infection withHelicobacter pylori(H.pylon) could be a causative agent of gastric diseases. The sources of H.pyloninfection and the transmission routes are still unconfirmed. The purpose of this study was to investigate the presence of H.pylonin denture plaque. Denture plaque was obtained from the fitting surfaces of dentures from 31 elderly inpatients. H.pylonwas detected by using polymerase chain reaction (PCR). A polymerase chain reaction (PCR) was designed to amplify the H.pylongenes encoding 16SrRNA and Urease A. Two patients were PCR positive for H.pylonin denture plaque.