Quantitative Analysis of Activated Glucocorticoid Receptors in Nasal Polyps

抄録

Objective: The purpose of our study was to measure the activated glucocorticoid receptor (GR) protein level in the nuclei of nasal polyp cells and compare the results in nasal polyposis patients with chronic rhinosinusitis alone and with chronic rhinosinusitis associated with asthma, before and after glucocorticoid (GC) therapy.
Methods: We used enzyme-linked immunosorbentassay (ELISA) techniques to quantitatively measure the activated-GR protein level in the nuclei of the nasal polyp cells. Nasal polyp tissues were obtained from patients with chronic rhinosinusitis alone and with both chronic rhinosinusitis and bronchial asthma. In the latter, polyp tissues were separately obtained before and after GC therapy.
Results: Our data showed no significant differences between the activated-GR protein level of the nasal polyposis patients with chronic rhinosinusitis alone and with both chronic rhinosinusitis and bronchial asthma before GC therapy. However, the activated-GR protein level was significantly upregulated after GC therapy in the patients with both chronic rhinosinusitis and bronchial asthma.
Conclusion: The result of our research clearly showed that GCs upregulated the activated-GR protein level in the nuclei of nasal polyp cells and that the upregulation is essential for the anti-inflammatory action of GCs.