Abstract
Lately, chromatography has come to be used for microestimation of matter. In this study, I divided conjugated fatty substance with the aid of magnesium oxide into cholinephosphatide and non-choline-phosphatide; next, by hydrolyzing the former, estimated the quantities of lecithin and sphingomyelin; then by hydrolyzing the latter, estimated that of cerebroside, and then by applying the Permutit method, estimated those of phosphatidylethanolamine and phosphatidyl-serine. While chromatography with magnesium oxide formerly required a considerable time for development, I resorted to mixing powdered glass (200 mesh) into the specimen in the 1: 2 weight ratio;thus working on the medium for such development, I succeeded in obtaining an efficient and perfect system for fractionation of fatty substance under which none of the fractions stuck to the adsorbent are lost.I also devised a system for causing the complete separation of non-choline-phosphatide from the adsorbent: first to dissolve MgO column in a liquid composed of equal quantities of 6N-HC1 and methanol, and then to extract fatty substance out of such solution with the use of ctloroform (CHC13). In this way, the five fractions of fatty substance i. e. lecithin, sphingomyelin, cerebrcside, phcsphatidyl-ethanolamine and phosphatidyl-serine are estimated in a series of continuous laboratory work.
