石綿およびベリリウムの肺胞マクロファージに及ぼす影響

抄録

Alveolar macropahges from rabbits were exposed in culture medium to asbestos, beryllium sulfate and beryllium oxide. The specific activities of the lysosomal hydrolases, acid phosphatase; β-N-acetylglucosaminidase; β-glucuronidase; and glycolytic enzyme, phosphohexose isomerase, were determined in the medium, whole cell homogenates, mitochodrial fractions and in the supernatant. These hydrolases increased significantly in the medium but not in the mitochondrial fraction of cells exposed to dusts. For the induction of enzyme release in vitro, a higher concentration of beryllium oxide than that of the other two dusts was requisite. Very cytotoxic effects on the cells were seen in the culture cell groups of both asbestos and beryllium sulfate.