Abstract
To standardize cytological bile juice preparation, we examined the following points and their usefulness using brush epithelial gallbladder cells from 15 cholecystolithiasis cases : (1) cytomorphological changes after cells were suspended in additive-free bile juice and the effects of different preservative fluids on (2) nuclear preservation and on (3) cell collection.
Brush cell nuclei in additive-free bile juice showed reduction and pyknosis within 5 minutes after cell suspension, with maximum change noted within 4 hours. Cell suspended in bile juice including CytoRich® or YM® fluid nuclei were well preserved up to 24 hours. A significant number of cells were collected using bovine serum albumin, Roswell Park Memorial Institute (RPMI) 1640 medium, 50% ethanol, CytoRich, YM, and Postsampler® fluids. Gallbladder cancer cell nuclei were well preserved after adding YM fluid to routine bile juice for 48 hours. In contrast, nuclear changes indicated poor preservation of brush cells or cancer cells collected in bile juice alone.
Preservation and cell collection results were good for alcohol-based fluids, indicating their effectiveness for use combined with routine bile juice and enabling accurate diagnosis.
