抄録
At the times of routine on the cytologic diagno sis in the field of gynecology we may sometimes encounter with difficulties in discrimination of cells from severe dysplasia and carcinoma in situ. In other words parabasal dysplastic cells and malignant basal cells are several times hardly distinguishable from each other. Resently the classification of parabasal dysplastic cells into three types (A, B, and C type) was proposed by Tenjin and coworkers. By investigating of frequency of these cells appe ared in cervical smears from above epithelial lesions, they made an attempt to find out the correlation of these cells with histologic typing. The purpose of the present study is to investigate and to analyze more objectively their theories.
One of the four populations used in this study was reserve cells, as representative of normal cells. The other populations consist of finely granular as well as coarsely granular dysplastic cells and epidermoid carcinoma cells.
All measurements were carried out by means of APAMOS version 2 on Zeiss's SMP-05 scanning microscopephotometer connected on line to small laboratory computer (PDP 12). A measuring spot size of 1.0×1.0 micron was used, and all measure ments were taken at a wavelength 550 nm. At first, digitized mirrorimages of individual cells were printed out and a cut-off point was established on the histogram of extinction values. In these mirror images the boundary between nucleus and cytoplsm was on the cut-off point methode drawn. These models fitting with boundary are statistically and mathematically analyzed and investigated.
The results may be summaried as follow:
1) Statistical assessment of the anti-smoothness measure of optical density in nucleus (Anti-Smoo thness Variance Measure and Anti-Smoothness Standard Deviation Measure) shows, that no significant differences between each cell population were recognized.
2) Assessment of complexity measure of chromatin contour: At first, the contour of different extinction levels was drawn. Let H denote the height of extinction level, S denote the area surrounded by contour and L denote the length of contour, then L2/S values of each extinction level (H) were obtained. Investigating L2/S values in a given cell, the value on height (H) of most complicated contour is appoximately at maximum of L2/S. It can be considered, that it is possible to predict the histologic type of epithelial lesion whichis present, when the distribution of the value H which maximizes L2/S and the Max.L2/S values of individual cells are investegated.
3) In the process of calculation for complexity measure of nucleus it may be possible to recognize mathematically shape and number of chromatin granules.
