抄録
The cytological diagnosis of malignancy is based on nuclear findings. Hematoxylin solution is used to demonstrate this nuclear detail. Fomula for making hematoxylin solution most often used for smears is. Harris.
Recently, harmful chemicals; Cd As, Hg, Cr, P, CN, PCB, etc., are limited in use. Harris hematoxylin solution contains plenty of mercuric oxide. We have to care about use of it.
Our present study was hematoxylin solution without harmful chemicals. Based on our results, two useful formulas for making hematoxylin solution were Gill and Lillie Mayer for Papanicolaou stain.