Genetic Transformation of ‘Kyoho’ Grape with a GFP Gene

Abstract

‘Kyoho’ grapevine (Vitis × labruscana Bailey) was successfully transformed by Agrobacterium-mediated transformation. Embryogenic calli (EC) were co-cultured with A. tumefaciens strain, EHA105 that harbors a green fluorescent protein (GFP) gene with the CaMV35S promoter. Kanamycin at 15 mg·L⁻¹ was suitable for isolating transformed EC from which three plants that express GFP fluorescence were regenerated. DNA analyses, including polymerase chain reaction (PCR) and PCR-Southern hybridization, confirm that these three plants contain sequences comparable to the GFP gene to indicate that it was successfully incorporated into the ‘Kyoho’ grapevine genome.