Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
ORIGINAL ARTICLE (ENGLISH)
Analyses of Expression Patterns of Ethylene Receptor Genes in Apple (Malus domestica Borkh.) Fruits Treated with or without 1-Methylcyclopropene (1-MCP)
Miho TatsukiAtsushi Endo
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JOURNAL FREE ACCESS

2006 Volume 75 Issue 6 Pages 481-487

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Abstract

1-Methylcyclopropene (1-MCP) strongly inhibits ethylene-mediated ripening effects. Since 1-MCP binds irreversibly to ethylene receptors, and ethylene sensitivity is recovered through the appearance of new ethylene receptors, it is important to characterize ethylene receptor genes to understand 1-MCP efficacy. We isolated three putative ethylene receptor genes from apple fruit and examined Md-ETR1, Md-ERS1, and Md-ERS2 expression patterns in 1-MCP-treated and -untreated fruit of ‘Orin’ and ‘Fuji’. In both cultivars, treatment with 1-MCP suppressed ethylene production and rapidly decreased expressions of Md-ERS1 and Md-ERS2, but Md-ETR1 mRNA was slightly reduced by the application of 1-MCP. One possible explanation for the high 1-MCP efficacy in apple fruits is that 1-MCP suppresses ethylene production and Md-ETR1 is expressed in 1-MCP-treated fruit. Md-ERS1 and Md-ERS2 mRNAs accumulated more abundantly in untreated ‘Fuji’, which has a long shelf life, than in ‘Orin’. Since ethylene receptors negatively regulate ethylene signaling, differences in expression levels between the cultivars might be reflected in differences in ethylene sensitivity.

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© 2006 by Japanese Society for Horticultural Science
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