1. With 12 species of fruit trees, the seasonal changes of sugar and polyhydric alcohol contents of twig barks and leaves were observed at certain intervals by a method of paper chromatography during a period from summer to winter. As the result of it, the content of polyhydric alcohol increased almost proportionally with that of sugar as the season advanced in all of species.
2. In apples, plums, pears, peaches and cherries, a considerable amount of polyhydric alcohol was contained both in summer and winter. The quantitative ratio of it to sugar was nearly 70% in summer and 43 to 73% in winter. In persimcoons and grapes, only a small amount of polyhydric alcohol was contained in summer, but it increased greatly in winter, the quantitative ratio of it to sugar being about 50%. In citrus fruit trees, the content of polyhydric alcohol was very low even in winter.
3. The kinds of sugar contained were almost the same regardless of the species of fruit trees, though the proportion of their contents differed markedly with the species. In every species, fructose, glurose, and saccharose were mainly contained. Raffinose appeared first in September or October and stachyose in November, increasing rapidly toward the end of December.
