Abstract
Morphological and cytochemical differences between susceptible and resistant varieties were surveyed to clarify the natures of the resistance on the chestnut trees to the gall wasps. Results were as follows.
1. Remarkable differences were found in the size of galls and wasps between susceptible and resistant varieties, and larvae were surviving not only in the susceptible but considerably in the resistant.
2. Nuclei, nucleoli and cytoplasm were rich in the divission layer of galls, but with increasing the resistance this inclination decreased.
3. In galls (esp. divission layer) of the susceptible, histone, indol and other protein reactions appeared intensively, concluding the protein synthesis.
4. Deposit of cellulose on the cell wall was poor in the susceptible galls (esp. divission layer).
5. Dehydrogenase and succinodehydrogenase were extremely active in the susceptible galls, but not at all or scarcely in the resistant.
6. The divission layer of the susceptible galls. exhibited intensive peroxidase and NADI-oxidase reactions. In the parenchyma tissues, peroxidase was strong in the susceptible but NADI-oxidase, reversibly, in the resistant.
7. Browning associating catechol tannins was occured at more than 50% in the relative resistant varieties and at beyond 90% in the heavy resistant. Polyphenoloxidase causing browning was slightly more active in the resistant.
