Abstract
The experiment was carried out to study the morphological and histochemical changes in the stem during the growth in Brassica species.
In the preceding report, the auther reported that as to the internode elongation at early stage of growth, there was no difference among all species and cultivars of Brassica used for studies but they were classified into four types after fourth leaf stage of growth: namely, 1). rosette type stem, 2). C-genome type stem, 3). Nigra type stem and 4). long vegetative growth type stem.
In this experiment, histochemical changes in the process of internode elongation of the four types of Brassica species were studies as to monosaccharides, polysaccharides, nucleic acid and lignins respectively.
The results were as follows: The elongation of hypocotyl occurred during the early stage from 4 to 6 th leaf stage, afterwards it stopped and lignification of the cell wall occurred in the four types. No essential difference was observed in histological observation, although morphological differences were recognized in four types of the internode elongation.
With all histochemical techniques used in this study, no difference was observed in these four types of internode elongation. The internode elongation and scape elongation were compared with histological and histochemically.
In despite of much differences in the rate of their internode elongation, no histological and histochemical differences were observed between the internode tissues and scape tissues in all varieties.
From these results, some discussion was made as to the internode elongation and scape elongation.