Abstract
1) The embryo culture technique was applied to the immature hybrid embryos for the purpose of enhancing their growth. The embryos used as materials were from 2 crosses between distantly related species or hybrids: L. longiflorum X L. 'Sugehime'and L.'Shikayama'X L. henryi.
2) The Murashige & Skoog medium was recognized as the most suitable among the three basic media for the culture of immature embryos when it was adjusted at pH 5.0 and supplemented with 20-40g/l sucrose. Higher sucrose concentrations than the above mentioned generally enhanced root growth and inhibited shoot growth.
3) The addition of 10-4-10-2mg/l NAA to the basic media was effective for the direct germination of immature embryos and the addition of BA tended to lead them to calluses in some degree. Higher NAA concentrations than the above mentioned promoted root growth and inhibited normal shoot growth, while the higher BA concentrations sometimes gave the reverse result to NAA.
