Abstract
In order to investigate the possibility of shoot formation from cultured tissues of strawberry fruits (receptacles), effects of age and seeds (achenes) on callus and shoot formation from cultured fruit tissues were studied.
1. When fruit tissues with seeds two and seven days after anthesis were cultured on the medium supplemented with 0 or 0.1mg/l BA+1 or 5mg/l 2, 4-D, white friable callus was produced around the basal part of the explant and grew rapidly. When the callus was subcultured on the medium supplemented with 10mg/l BA+ 0.1mg/l 2, 4-D, shoots were formed.
2. When fruit tissues without seeds two and seven days after anthesis were cultured on the medium supplemented with 1 or 5mg/l BA+0.1 or 1mg/l 2, 4-D, green firm callus was produced from the entire surface of the explant and then shoots were formed from this callus.
3. When fruit tissues with seeds seven days after anthesis and fruit tissues with or without seeds twelve days after anthesis were cultured on the medium supplemented with 0.1 or 5mg/l BA+1 or 5mg/l 2, 4-D, pale green firm callus composed of very small cells appeared around the basal part of the explant, but no shoots were formed on this type of firm callus.
4. When fruit tissues with or without seeds seventeen days after anthesis were cultured, cell enlargement was observed but no callus was formed regardless of the concentrations of the growth regulators supplemented to the medium.
5. When shoots formed on callus were separated and subcultured on the basal medium without growth regulators, roots were formed within two weeks and young plantlets were established.
