Adventitious Bud Formation from Excised Hyacinth Bulb Scale in vitro
1978 Volume 46 Issue 4 Pages 501-508
Details
1978 Volume 46 Issue 4 Pages 501-508
The present study was carried out in order to pursue some medium constitution and plating method and also the meaning of the difference in the localization about the adventitious bud formation of the hyacinth bulb scale culture.
Commercial varieties, i.e. ‘La Victoire’, ‘Delft Blue’, ‘Lady Derby’, and ‘Jan Bos’ were used.
The following results were obtained:
Hyacinth bulb scales cultured on Murashige and Skoog basic medium, same basic medium plus 0.001 to 0.005mg/l, 2, 4-D and also containing IAA and BA 0.5mg/l respectively showed good adventitious bud formation. In regard to the concentration of 2, 4-D on organogenesis, adventitious bud formation was observed between 0 to 0.1mg/l, especially at very low concentration, and adventitious root formed between 0.001 to 0.5mg/l, especially at 0.01mg/l, whereas callus formed when the concentration was considerably higher, especially at 0.5mg/l.
The adventitious bud usually formed on the epidermis of the outside of the scale in hyacinth and this feature was contrary in the case of lily where the bud formed on the epidermis of the inside of the scale.
By the histological observation of adventitious bud formation processes there seemed to be initial cell or cells in the epidermis. However, when the epidermis alone, which was stripped from the scale, was cultured, it showed no bud formation by itself on all the medium used, and the remaining scale without the epidermis formed callus or adventitious root.
When the method of plating was altered, whether the outside epidermis was on the upper side or bottom side, the bud forming response differed in connection with the constitution of media. Therefore, the localization of endogenous auxins and cytokinins would have an important role in the adventitious bud formation on hyacinth bulb scales, and it was considered that the polarity would exist morphogenetically between the outside epidermis and the inside epidermis, and it can be broken down by some inducing substance.
