Studies on Vegetative Propagation of Tulip

II. Formation and Development of Adventitious Buds in the Excised Bulb Scale Cultured in vitro

Abstract

In the present work, effects of 2, 4-D and kinetin of various concentrations in the medium on adventitious bud formation and its subsequent development in the excised scale of tulip was studied in relation to the morphogenic potential of differently aged bulbs. The concentration and combination of 2, 4-D and kinetin with other ingredients in the culture media were modified to find out effective compositions of media that bring about the differentiation of adventitious buds. When excised scales of bulb, cv. ′Apeldoorn′, were cultured on Murashige-Skoog′s medium, the most suitable combination of auxin combined with kinetin was 1.0-1.4mg/l 2, 4-D for this purpose and that of cytokinin combined with 2, 4-D was about 3mg/l kinetin.
The initiation of adventitious buds in the scales cultured in vitro was greatly affected by the age of mother bulbs. The cultures failed to initiate bud formation unless cultured explants were taken from the suitably aged bulbs, even when the other conditions were favorable to it. August for ′Apeldoorn′ and May for T. hageri were the most suitable time for sampling each bulb scale culture.
Lower concentration of growth substances in the medium were more effective to the subsequent development of newly formed buds when cultured in the light for additional 3 months. As a result, small bulbs were formed eventually at the base of the adventitious bud.