1981 Volume 49 Issue 4 Pages 497-504
Experiments were carried out to obtain further information about self-incompatibility in Hyuganatsu, Citrus tamurana hort. ex Tanaka. The results obtained were as follows.
1. Using disc electrophoresis, sequential changes in buffer soluble proteins and peroxidase isozymes in self-pollinated pistils were compared with those cross-pollinated with Hassaku, Citrus hassaku ex. Tanaka. Remarkable differences in the band distributions of buffer soluble proteins were observed in samples from stigmas, styles and ovaries at any time within 24 hours after pollination. Differences in peroxidase isozyme distributions between self-and cross-pollinations appeared first in the ovaries, second in the styles and last in the stigmas.
2. Several treatments were tried in search of an effective method of overcoming self-incompatibility. Self-pollination after smearing the stigma exudate of mature Hassaku flower buds onto the stigmas of immature Hyuganatsu flower buds, half the length of mature ones, was found to be the most effective of the treatments tried. Repeated pollination of immature flower buds for 4 continuous days was nearly as effective as the preceding treatment. With both treatments, a number of small seeds, 1/3 size of normal ones, developed; these might be expected to be seedless triploids.
