Abstract
Regeneration of intact plants of Zenkoji grapevine was established by meristem tip culture for the purpose of obtaining virus-free plants. Half strength Murashige and Skoog (1962) Medium supplemented with 0.1mg/liter NAA, 1.0mg/liter BA, 0.5mg/liter kinetin, 4.0mg/liter adenine, 30g/liter sucrose and 6.0g/liter agar (Medium I-2) was optimal for the greening and enlargement of inoculated meristem tips. For the subsequent growth, monstrous leaf formation and succeeding multi- shoots formation, it was necessary to substitute 0.2mg/liter IAA for 0.1mg/liter NAA (Medium II-1). The further development of shoots, opening and growth of small leaves, was better on the Medium II-1 but with BA and sucrose concentration reduced by half (Medium II-4). Rooting was induced on Galzy (1964) medium supplemented with 0.1mg/liter NAA (Medium III-1) and the further plant development was better on the same medium but without NAA (Medium III-2). Plants were easily established in soil.
Repeated multiplication was also achieved by transplanting the fully developed shoots on a half strength MS (1962) medium supplemented with 0.2mg/liter IAA, 1.0mg/liter BA, 0.5mg/liter kinetin, 4.0mg/liter adenine, 15g/liter sucrose and 6.0 g/liter agar (Medium II-2) followed by Medium II-4.
