1984 Volume 53 Issue 1 Pages 6-12
Some experiments were carried out to obtain further informations concerning the effect of bud pollination on overcoming the self-incompatibility of Hyuganatsu, Citrus tamurana Hort. ex Tanaka. The results obtained are as follows.
1. By means of chromatofocusing analysis, physiological differences between mature and immature flower buds were clarified with respect to their proteinous components in pistils. As to soluble proteins, glyco-proteins and peroxidase isozymes in stigmas, styles and ovaries, there existed clear differences between mature and immature flower buds. But, no remakable differences on esterase and alkaline phosphatase isozymes were observed between the two kinds of flower buds.
2. Scanning electron microscopic observation revealed that stigma exudate of immature flower buds was less abundant than that of mature ones. It also showed that covering of Hyuganatsu pollens by exudate on stigmas of immature flower buds was not so rapid as that on the stigmas of mature flower buds. According to fluorescent microscopic observation, the pollen germination on the stigmas was rather delayed in immature flower buds as compared with mature ones. Nevertheless, the pollen tubes grew smoothly through the styles of immature flower buds, and entered ovaries 7 days after pollination.