Abstract
The transplanting to soil of Lilium rubellum Baker bulblets cultured in a Murashige and Skoog′s medium (see table 1) was investigated.
Leaf emergence from in vitro cultured bulblets was inhibited after the transplanting into soil without any previous treatment. Temperature treatment (8°C or less) prior to transplanting had the effect of stimulating leaf emergence from the bulblets. Bulblets, chilled at 4°C for 12 weeks, accomplished their leaf emergence 20 days after transplanting, but leaf emergence from bulblets chilled for 10 weeks or less depended on the growth temperatures after transplanting, suggesting that chilling at 4°C for 12 weeks was required to completely break the bulblet dormancy.
GA3 was applied to non-chilled, partly chilled and fully chilled bulblets by immersing and shaking them in distilled water containing GA3 at a concentration of more than 250mg/l. This treatment stimulated leaf emergence even in non-chilled bulblets after transplanting, but it was less effective for breaking dormancy when compared with the chilling treatment (4°C for 12 weeks). In partly and fully chilled GA3-treated bulblets, the GA3 treatment appeared to have the effect of reducing and substituting for the chilling period in respect to rate and percentage of leaf emergence and in development of bulblets after transplanting.
