Abstract
Medium composition for successful shoot tip culture to multiply the suckers induced from a promising dwarfing rootstock (Prunus japonica THUNB.) on which certain peach cultivars had been grafted, was investigated with respect to cytokinin and auxin levels under continuous light at 25°C.
Shoot tip cultures of P. japonica were established to proliferate shoots on 1/2 or 1/3 strength modified Murashige and Skoog (MS) medium. Sub-cultures treated with BA 0.5mg/l produced elongated shoots suitable for rooting.
Sorbitol was effective as a carbon source for shoot multiplication during culture.
Elongated shoots were incubated on media with and without auxin for 30 days. Rooting was better when shoots dipped in IBA solution (50ppm) were then incubated on a medium without hormones. NO3/NH4 mole ratio based on total N in the MS medium was studied.
The best combination for shoot growth was 1/3 strength MS when both forms were present in the same ratio.
