Abstract
An automated system was developed for measurement of the rates of O2 uptake, CO2 output, and C2H4 evolution by fruit and vegetables simultaneously by use of a microcomputer and three gas chromatographs. The microcomputer was also used to regulate sample temperature and prepare the gas mixtures of 22, CO2, N2, and C2H2 in various concentrations and to regulate the flow rate. Changes in the rates of O2 uptake, CO2 output, and C2H4 evolution associated with the ripening of banana fruits and the wounding of winter squash fruits were measured to check the performance of the system. The stimulation of respiration by exogenous C2H4, and the inhibition of respiration and C2H4 evolution by a high concentration of CO2 were measured with sweet potatoes and peaches, respectively. The rates of change of the gases corresponded to well-known physiological responses to given conditions of flow gas in every sample tested. CO2 output from the sample materials could be measured even in the flow gas containing a high concentration of CO2, up to 60%. C2H4 evolution could be measured directly in the presence of external C2H4. Analysis of the response of fruit and vegetables in gas metabolism, to various gaseous environments of the system was satisfactory.
