Abstract
Explants excised from an internode, immature and mature leaves, petiole, immature seed and fruit, and an in vitro-differentiated root of tara vine (Actinidia arguta Planch.) were cultured on Miller's medium containing sucrose (30 g•liter-1) and gelrite (2 g•liter-1), and 15 combinations of BA (0, 1, 10 μM) and NAA (0, 0.1, 1, 10, 100 μM).
Callus formed on all explants except that from a mature leaf. Adventitious buds formed on the surface layer of the calli which were induced from the cortex of an internodal segment. After 60 days of culture, shoots emerged from the adventitious buds. Shoot formation was suppressed with an increase in NAA concentration, but this suppression tended to be reversed by the addition of BA. When transferred to the medium supplemented with combinations of 0, 2, 4, 6, 8 and 10 μM NAA and 1 and 10 μM BA, the in vitro-differentiated shoots formed roots in a medium with 4 μM NAA and 1 μM BA, and regenerated into plantlets after 45 days of subculture.
For acclimation, the plantlets were transplanted to a synthetic soil (soil : vermiculite, 4 : 1, v/v) ; nearly all survived, becoming lianoid 40 days later.
