Abstract
Substance (s) inhibitory to ACC-dependent ethylene production in the alcohol-insoluble solids (AIS) fraction from peach fruit was assessed in apple fruit discs.
The supernatant from AIS digested with pectolyase Y-23 in distilled water inhibited ethylene production in apple fruit discs. Separation of the supernatant on a PD-10 column indicated that the inhibitory substance (s) was associated with the low molecular weight fraction. The inhibitory effect of the supernatant was lost upon dialysis against distilled water. The inhibitory fraction appeared as a single entity (Rf =0.44) when separated by thin layer chromatography. Inhibitory substance (s) was also released by macerozyme and cellulase digestion or by autocatalytic digestion of freshly prepared peach fruit homogenate. These data indicate that peach fruit may release a substance (s) which inhibits ethylene production.
