Abstract
Bulblets of Lilium rubellum Baker were cultured for 16 weeks in a 20-, 30- or 40-ml liquid medium renewed 0, 1, or 3 times. The bulblets were assessed for growth and rot infection.
1. Bulblets with fresh weight of more than 600 mg were obtained by using a 20- or 30-ml medium with 3 renewals, or 40 ml with one renewal.
2. Bulblets frequently turned brown when they were cultured in a 30- or 40-ml medium with 3 renewals; they frequently rotted both during a cold treatment and after transplantation.
3. Bulblets cultured in a 20-ml medium did not rot after a cold treatment irrespective of the number of medium renewals; they grew well after transplantation to the greenhouse.
4. Bulblets cultured in a 20-ml medium with 3 renewals most frequently developed into plantlets with elongated axes 15 weeks after transplantation.
These results indicate that the 20-ml medium with 3 renewals appears to be most suitable for bulblet culture of L. rubellum in a liquid medium.
