Studies on the Propagation of Fritillaria camtschatcensis Ker-Gawl. by in Vitro Culture : I Characteristics of Sprouting, Leafiness, Flowering and New Bulb Formation from Mother Bulb, and Bulblet Formation from Small Globule-scale

Abstract

Wild black lily bulbs were planted in pots and grown for 8 months in a glasshouse. The bulbs produced scapes with 1∿2 terminal flowers and 5∿6 leaves with a verticillate phyllotaxis. Tow new bulbs were formed on the mother-bulb as it was consumed and eventually rotted. Morphological characteristics matched those of Fritillaria camtschatcensis Ker-Gawl. Small globule-scales of the bulbs were excised and cultured on MS-media without plant hormones in the dark or under light of 40μmol·m^-2·sec^-1 at 15, 20, and 25°C for 8 weeks. The optimum condition for bulblet formation was a MS-medium containing a combination of 0.1 mg·liter^-1 NAA and 0.1 mg·liter^-1 24-epibrassinolide (EB) at 20°C in the dark.