Abstract
Growth rates of Phalaenopsis callus differed depending on the volume of media. Whereas callus growth on 5-ml of medium slowed after 8 weeks of culture, that on 10- and 20-ml of media grew slower after 10 and 12 weeks, respectively. Taro extract (TE), potato extract (PE), coconut water (CW), and apple extract (APE) enhanced callus growth but the effects depended on the quantity of each supplement. TE was the best additive tested on callus growth when 50 to 200 ml·1-1 were added. Banana homogenate (BH) and peptone had little effect on callus growth, whereas sweet potato extract (SPE) proved inhibitory at all levels tested. The effects of TE, PE, and CW depended on each callus. No synergistic effects of CW, TE, PE, and APE were observed.
