Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Efficient Production of interspecific Hybrids between Spiraea thunbergii Sieb. ex Blume and S. japonica L. fil. through Ovule Culture
Masahide IizukaNobuhiro KudoYasuo KimuraIsao Ogiwara
Author information
JOURNAL FREE ACCESS

2003 Volume 72 Issue 4 Pages 347-351

Details
Abstract
To establish an efficient means of regenerating plantlets via ovule culture from an interspecific cross between Spiraea thunbergii Sieb. ex Blume and S. japonica L. fil., the effects of concentrations of macro-elements in the MS medium and sucrose and the sampling time of ovules were investigated. Survival rates of ovules and normal plants, on the MS medium in which macro-elements were diluted to 1/2 or 1/4 were better than those cultured on standard, undiluted MS medium. The high concentrations of macro-elements increased the rate of blighted plants. Hybrid plantets developed on 1/4 MS medium supplemented with 3% sucrose. Although the frequency of albino plant was not influenced by the concentration of macro-elements in the medium, their rate increased as ovules were cultured in media high in sucrose. When ovules were excised 5 to 22 days after pollination and cultured in 1/4 MS medium supplemented with 3% sucrose, the percentages of normal plants that developed ranged from 1 to 31. When ovules in their differentiation and enlargement stages were excised from 14 to 19 days after pollination and culture, the frequency of normal plants ranged from 25 to 31%. Thus, the success rate of obtaining interspecific hybrids between Spiraea thunbergii Sieb. ex Blume and S. japonica L. fil., was optimum when hybrid ovules were excised from 14 to 19 days after pollination and culture on 1/4 MS medium supplemented with 3% sucrose.
Content from these authors
© Japanese Society for Horticultural Science
Previous article Next article
feedback
Top