Plant Regeneration from Suspension Cell Culture of Delphinium.

Abstract

Embryogenic calli were induced from cotyledons of 'Pacific Giant' Delphinium hybridum (white line) in MS medium containing 1.0 mg-liter^-1 2, 4-D, 0.1 mg-liter^-1 BAP and 30 g-liter^-1 sucrose. Embryogenic cell suspension cultures were established from these embryogenic calli by using a liquid N6-based medium (N6 inorganic salts and MS organic constituents), containing 1.0 mg-liter^-1 2, 4-D, 1.0 mg-liter^-1 TDZ and 30 g-liter^-1 sucrose. The cell aggregates increased about eight-to ten-fold after one week of culture under fluorescent light at 25°C. When suspension cell clusters were transferred to a N6-based medium that lacked plant growth regulators, many somatic embryos were formed. When these somatic embryos were transferred to the same medium, the shoots that differentiated were transferred to 1/2 MS medium to root. About 20 plantlets were produced from 1 g of suspension culture.