抄録
Four Moncclonal Antibodies (MoAb) were produced by hybridomas fusing immunized (by human neuroblastoma) mouse speen cells with mouse myeloma cell line p3-X63-Ag8653. These MoAb showed restricted reactivity for human neuroblastoma cell lines by ^<51>Cr release cytotoxicty assay in vitro, but did'nt react with human melanoma, mouse neuroblastoma and fibrosarcoma cell lines. In the immunohistochemical experiment of indirect immuncfluorescence using these MoAb, they reacted specifically with only neuroblastoma cells. By Avidin-Biotin-Complex (ABC) technique, paraffin section of human neuroblastoma tissues stained positively, but some of them cross-reacted with rhabdomyosarcoma specimen. This result suggests that these MoAb may by particularly useful reagent for the clinical diagnosis of human neuroblastoma.
