抄録
With the use of chromogenic substrate (S-2251), rate assay (kinetics) and end point assay of enzyme activity of human plasmin and human plasminogen, euglobulin and plasma by SK or UK were studied using by auto-analyzer (Hitachi-Co., type-703).
The chromogenic substrate was enzymatically good substrate for plasmin and Km value of plasmin was 7.6×10-4mM.. When plasma was activated by SK, the highest value of kinetics was seen at initial time but UK had lag time. From these results, it is suggested that even with the use of small dose of substrate (0.1uM), auto-analyzer could be applied to measure fibrinolytic activity of human plasma (0.05ml) activated by SK (500U) on kinetics.
