抄録
We describe a highly sensitive enzyme immunoassay system by using β-D-galactosidase from Escherichia coli as a label for quantitation of fibrinogen and its degradation products. The minimum detectable concentrations of fibrinogen, fragment D and fragment E were 0.6, 0.06 and 0.2ng/ml of reaction mixture, respectively. The cross-reactions of the assay system for fragment E with fibrinogen and fragment X, Y and D were 5, 8, 56 and 0%, respectively. The assay system for fragment D did not cross-react with fragment E, but crossreacted with fragment X, Y and fibrinogen (38, 10 and 15% respectively). The assay system for fibrinogen did not cross-react with fragment D and fragment E, but cross-reacted with fragment X and Y.
Fractionation of the serum sample with Sephadex G-200 column chromatography made it possible to quantitate the fragment D and fragment E, specifically in serum samples.
