ウロキナーゼの腸管吸収と血中線溶活性化

抄録

Following intrarectal administration of high molecular weight urokinase (HMW-UK; mol. wt. 53, 000, 124, 000IU/mg protein) or its functionally-active heavy chain (H-chain UK; mol. wt. 31, 800, 212, 000IU/mg protein) to mice, prolonged activation of plasma fibrinolysis was observed. The plasma levels of pyro-Glu-Gly-Arg-pNA amidolytic and euglobulin fibrinolytic activities reached a maximun (42.8±5.6nmoles/ml and 10.6±7.1mm/0.03ml, respectively) at 1 to 2hr after the administration of 300IU of either enzyme per mouse. Thereafter, both activites declined slowly. Absorption of the UK was confirmed by pooling the fractions which were eluted by 6M urea buffer following [N^α-(ε-aminocaproyl) -DL-homoarginine hexylester]-Sepharose affinity chromatography. Although the eluted fractions were shown to form a precipitation arc with appropriate antisera by immunodiffusion, the neutralization was not complete and approximately 40% of the activity remained. Activation of plasma fibrinolysis may not be due only to UK absorption but also be related to some enzyme indirectly released.