A Chinese herb, Chi-shao (CS: a kind of Paeoniae Radix) was previously reported to inhibit plasmin. In this note, the active component was purified from CS extract: When active solution obtained by aceton fractionation (33-55%, v/v) was chromatographed with a Sephadex G-50 column, a target ingredient was eluted forming a broad peak with large tailing which indicated weak adsorption to the Sephadex bed. By the re-chromatography in the presence of a detergent, the component was eluted without tailing. The resulting chromatogram showed that the molecular weight of the component should be about 1, 200.
The purity was increased by almost 1, 000 times from the initial extract.
As to the mode of plasmin inhibition, combined effect of CS extract and synthetic inhibitor, tranexamic acid (tA) was examined: When an amidolytic assay (S 2251) was used, to interrupted the inhibition of plasmin by CS, whereas in fibrinolysis, CS and to were additive to each other.
