Abstract
A radioimmunoassay technique for the fragment Eneo-antigens in plasma was developed. Specific antibody to the Eneo-antigen was obtained from anti-E rabbit serum through a fibrinogen-Sepharose column. There was no interference on binding between Eneo-antibody and 125I-E even though hundreds mg of fibrinogen were present in a sample to be tested. Therefore, the method was applicable to plasma sample, permitting quantitation of the E-related neoantigens more than 1ng/ml. On the Eneo-antigenicity, electro-blotting study showed that the products of fragments X and Y which were generated in the early stage of fibrinolysis were bearing the antigenicity. Among the chains of fibrin and fibrinogen molecules, β and γ chains were strongly suggested to be the loci of Eneo-antigenic sites.
