抄録
The effect of thrombin, ADP or ergonovine on the release of plasminogen activator (PA) and prostacyclin was studied in the isolated perfused dog heart with or without coronary injury.
The PA activity of the perfusate was estimated by fibrin plate method. Thrombin induced dose-dependent PA release from the perfused heart, while DFP-thrombin gave no PA release. ADP induced weak PA release, but ergonovine, induced no PA release.
The local fibrinolytic activity was studied with fibrinolysis autography. After the administration of high-dose of thrombin into the perfused heart, the local fibrinolytic activity in the left anterior descending (LAD) branch of coronary arteries was decreased, while the activities in the great cardiac vein and small vessels of left ventricle were not altered.
6-keto-PGF1α in the perfusate was determined by radioimmunoassay. There was no detectable 6-keto-PGF1α (<50pg/ml) in the perfusate before and after the administration of the drugs.
Intimal injury was induced with balloon catheter in the LAD. The dogs were killed at 1 or 4 weeks after injury. The patterns of PA release from the injured heart were similar to those from the heart without injury. But the local fibrinolytic activity in the injured LAD was lower than that in the LAD without injury.
