抄録
Using monoclonal antibodies to FDP-D domain structure which were obtained in our laboratory, a method for quantitative determination of plasma FDP was developed without interference of fibrinogen present in plasma. The method was based on ELISA technique, combining β-galactosidase as a labelled enzyme and FDP-D coated polystyrene ball as a immunosorbent. When FDP-D was used as a reference, sigmoidal standard curve was obtained ranging 0.25 to 64μg/ml. Coefficients of variation in both intra and inter assays were 5 to 10%. Normal value by this method was below 0.75μg/ml. Correlation of FDP concentration between plasma and serum was good (γ=0.97), but not so good between our ELISA method and conventional Latex agglutination method (γ=0.60). The application of this method seems to be useful to clinical assays of plasma FDP concentration.
