カラゲニン誘発炎症に伴って増強されるマウス脾のプラスミノゲン分解活性

抄録

The variation of spleen plasminogenolytic activity was investigated using a carrageenan-induced inflammation in mice. Immuno-blotting method using antiserum for human plasminogen was adopted for the observation of plasminogenolysis by the spleen extract with 2M KSCN. The plasminogenolytic activity was detected even in the normal spleen extract. The remarkable enhancement of it in the spleen from the 6th to the 10th day after the carrageenan injection followed the increases of plasma fibrinogen and serum FD (g) P levels. The hydrolysis of plasminogen by the spleen extract made fragments of 55kDa, 35kDa and 28kDa, further the 55kDa fragment changed to the small one. These plasminogenolytic activities were completely inhibited with diisopropyl fluorophosphate, soybean trypsin inhibitor or α₁-trypsin inhibitor, but not inhibited with aprotinin. The variation of the spleen plasminogenolytic activity after the carrageenan injection and these effects of proteinase inhibitors for the activity were similar to the fibrinolytic activity in the spleen. These data show that the plasminogenolytic activity in the spleen was derived from a same proteinase as fibrinolytic activity.