異なる組織プラスミノーゲン活性化因子標品のフィブリン平板法による活性測定法上の問題点

抄録

To study the susceptability of fibrin plate method for determination of t-PA activity, the activity of melanoma t-PA (mt-PA) was compared with that of recombinant t-PA (rt-PA) using the International Reference Preparation for urokinase as a standard.
The dose-response lines of t-PA and urokinase were not parallel. Unexpectedly, in some cases, those of mt-PA and rt-PA were not parallel. The specific activities of them changed with the location on the urokinase standard line at which the t-PA was read and with experimental conditions.
This data suggested that the fibrin plate method was not admissible to compare the activities among t-PA preparations, derived from differnt sources.