It has suggested that hypercoaglability was induced activated factor VII by tissue factor in the estrinsic pathway from malignant tumor. Procoaglant activity may relate with tumor gorwth and development of metastasis. The procoaglant activity of four human cancer cells and of culture supernatant was measured by a one-stage plasma recalcifiation assay. The cell suspension of LK-2, LK-17, LK-52 and HLC-1 was shorter markedly the recalcifiation time of normal human plasma, not of factor VII deficient plasma. The cultre suspension of LK-2 was longer markedly the recalcifiation time of normal human plasma. The culture suspension of LK-17 was shoten markedly the recalcifiation time of normal human plasma, not of factor VII deficient plasma. The culture suspension of LK-52 was shoter the recalficication time of both normal human plasma and factor X, of factor VII deficient plasma. These Beta incident that LK-52 cancer cell line products a directer activater of coagulation facter X in cluture medium.
