培養ヒト子宮内膜線維芽様細胞の plasminogen activator とその inhibitor 産生に及ぼす性 steroid hormone の影響

抄録

The endometrium is a site of high fibrinolytic activity associated with menstruation, nidation of ovum and wound healing. To investigate the fibrinolytic system in the endometrium and the influence of sex steroids on it, tissue type plasminogen activator (t-PA) antigen, plasminogen activator inhibitor 1 (PAI-1) antigen and PA activity in the conditioned medium of the endometrial fibroblastoid cells (EF) cultured in the presence of these hormones were assayed. The growth rates on the fibrin gel substrate were also assayed.
t-PA synthesis and PA activity were enhanced by estrogen. PAI-1 synthesis was increased and PA activity was suppressed by progestrone (P), estradiol (E₂)+P, testosterone, dexamethasone and indomethacine. The cells were well proliferated on the fibrin substrate and no aggregation were observed in the case of high level PAI-1. E₂ brought about cell aggregation and cessation of growth due to the substrate degradation accelerated by t-PA.
It was suggested that the fibrinolytic system in the endometrium was controlled by sex steroids through the balance of PA and PAI synthesis.