ラテックス凝集法によるプラスミン-α₂-プラスミンインヒビター複合体の迅速測定法の臨床的有用性に関する検討

抄録

Plasma levels of plasmin-α₂-plasmin inhibitor complex (PPI) were measured by a rapid semi-quantitative method based on the latex agglutination immunoassay in patients with a variety of diseases such as hematological malignancies, infection, thrombotic disease and disseminated intravascular coagulation (DIC). As compared with healthy subjects, plasma levels of PPI were markedly elevated in the patients with DIC. In addition, some patients with thrombotic disease and hematological malignancies had high PPI levels. Serial determinations of PPI demonstrated a dynamic fluctuation of PPI during the course of DIC and during fibrinolytic therapy. On the whole, PPI values obtained by this assay were correlated well with those measured by an enzyme-linked immunosorbent assay. In addition, the plasma PPI level was correlated positively with concurrently assayed FDP and D-dimer levels, and negatively with plasma levels of fibrinogen, α₂-plasmin inhibitor and plasminogen. No correlation was found between the PPI level and antithrombin III, protein C or thrombin-antithrombin III complex values. These findings indicate that a rapid measurement of the plasma PPI level with this method would be useful for the assessment of activation of fibrinolysis in these disease states.