抄録
On the preparation of cryoprecipitate, the following conditions were considered as main factors for the good yield of factor VIII.
1. Temperature during the centrifugation of thawed plasma: The thawed plasma should be centrifuged at low temperature nearthe freezing point of plasma.
2. Temperature for freezing: The plasma should be frozen as quickly as possible at low temperature.
3. Condition of thawing: The plasma should be thawed slowly at low temperature in the ice box.
4. Period of the preservation of separated plasma: The plasma should be frozen as fast as possible after the separation from the blood cells.
5. Period of the preservation of frozen plasma in the freezer: The frozen plasma should not be left alone for a long time and it is recommended for the plasma to be thawed within four days.
6. PH range of plasma: PH of the plasma should be adapted from pH 7.25 to pH 6.75. The temperature during centrifugation for the separation of plasma was considered to affect a little on the yield of factor VIII and the temperature during preservation of frozen plasma not to affect very much on that, if temperature is under -20°C.
