The frequency of anti-E antibody formation has a very important problem especially in Japanese, and it has been studies in our laboratory since 1981. The anti-E positive specimens were found in 10 of 250 (4.0%) Rh(ee) recipients who received more than 10 units of blood. On the other hand, the frequency of anti-E formation was only about 2% in all Rh(ee) recipients in cluding cases received less than 10 units of blood. In the latter series, almost a half recipients were transfused with less than 10 units. And in general, the incidence of Rh(ee) recipients who receive E-positive blood in random transfusion is presumed to be about 25% in Japanese.
Consequently it is likely that the frequency of anti-E antibody formation is approximately 0.4-0.5% in all transfused recipients in Japan.