Abstract
It has become important to exclude adult T cell leukemia virus (ATLV) positive blood products for blood transfusion, because ATLV causes ATL, and ATLV is infected by the transfusion of ATL associated antibody (ATL-Ab) positive blood.
For purposes of massive screening of ATLV positive blood products, we improved two kinds of ATL-Ab screening method using indirect immunofluorescence assay (I. F.) and enzyme immuno-assay (E. I. A.).
I. F. which uses acetone fixed ATL cultured cells on slide glass as target cell, were performed using 50 well slide glass, multiple dispensor and serum diluter. This method increased the number of samples to be tested in a day.
E. I. A. using ATLV fixed U-plate were tested with shorter incubation time (30′) after adding test serum and alkaline phosphatase conj. anti human IgG. This rapid procedure also clearly discriminated ATL-Ab positive and negative serum for the screening of ATL-Ab.
