Abstract
Optimum centrifugation conditions for the preparation of platelet concentrate have been investigated from the aspects of quality and function of platelets including recovery, leukocyte contamination, pH, size distribution, aggregability, hypotonic shock response, shape change, adhesiveness and ATP release.
It was found that optimum results were obtained by centrifugation at the speed of 2, 000 rpm (1136×g) for five minutes in the preparation of platelet rich plasma, and at the speed of 3, 200rpm (1908×g) for five minutes in the preparation of platelet concentrate. The preparative protocols indicated greater effect on the quality of product in the first centrifugation of whole blood than in the second process.
