Abstract
The detection of anti-granulocyte allo-antibodies by two methods of flowcytometric immunofluorescence (FCM-method) and ATP-bioluminescence (ATP-method) is described. Granulocyte allo-antibodies were detected with high sensitivity in the sera of transfused patients by these two methods. The patients with high titer of granulocyte allo-antibodies had been showing post-transfusion reactions with fever at every transfusion. These methods were proved to be a rapid, reliable technique, which may be applied in routine screening of granulocyte allo-antibodies for a large number of clinical samples. FCM- and ATP-method are different in its principle of detecting reaction and the results were not always coincident. The detecting rate of antibody was higher in the FCM-method than in the ATP-method, however, a few antibodies were detected not by FCM-method but by ATP-method. For the improvement of detecting efficiency, combined screening with FCM- and ATP-method is desirable.
